NIS-Elements

NIS-Elements

Image Analysis System

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The EDF module allows you to combine existing Z-stack images into one focused image picking the focused regions from each frame and merging them together. An algorithm aligns the sequence to ensure correct fitting of pixels even for images acquired via macro optics, where objects are slightly shifted or scaled during the z-stack acquisition.

EDF

After the focused image is created, there are three ways how to observe the result: