NIS-Elements

NIS-Elements

Image Analysis System

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Live Cell Acquisition


Advanced ND Acquisition

The Advanced ND Acquisition module enables to define advanced ND experiments.

Different ND Acquisition Types (Z - 3D model, Timelapse)

It is possible to define: a complex experiment which can hold more timelapse acquisitions, each of them can contain different multi-point experiments. Next you can define as many ND sequences as you need, you can switch to another optical configuration, run a macro or command, etc., in each of the points of the multipoint experiment.

Triggered Acquisition

The triggered acquisition in NIS-Elements is the process of acquiring images where some actions during the acquisition are controlled solely by hardware (TTL and analog signals, waveforms) without the software interference. Connection generally requires cables, break-out boxes and/or specialized DAQ cards or cameras. As a result, higher frame-rates can be achieved. There are the following common applications of the triggered acquisition:

Multi-excitation

A camera triggers a wavelength switcher

Piezo Z

A camera triggers a piezo Z stage

Multi-excitation + Piezo Z

A combination of the above mentioned applications - a camera triggers a Piezo Z stage and a wavelength switcher.

Note

There are also other applications such as multi camera systems where the master camera triggers slave cameras, but it exceeds the scope of this tutorial (see the Dual Andor Handbook for further info on dual cameras systems).

Incubation

The Incubation module enables controlling of incubation systems.

  • Temperature and gas concentration is monitored and controlled using separate control panels.

  • The module enables to set up experiments with continuous imaging phase which go on under certain conditions (heating/cooling, gas concentration in the incubator's atmosphere, etc.).

  • You can flexibly combine the experimental phases. For example, when a set temperature is reached, a custom macro is executed, or the image is being captured. The other possibility enables to control the incubator by the course of experiment: when a predefined event occurs, the temperature or gas concentration is automatically reset to a new target value, etc.

  • Experimental conditions are recorded and saved along with the image file. So they can be used for further analysis of the obtained results.

TTL/Analog IO

Along with specialized hardware equipment, the TTL/Analog IO module enables to either:

  • control experiment events from outside of NIS-Elements

  • give feedback by generating an electric signal

With this ability, you are able to make your experiment really automated. You can for example:

  • Control Gas/Temperature inside an incubation chamber

  • Start capturing a time-sequence conditionally (e.g. when temperature is above certain value)

  • Communicate with any custom peripheral device.

  • etc.