Image Analysis System

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Document structure

NIS-Elements™ uses a sophisticated image documentation structure making it possible to achieve non-destructive archiving of image data including annotation (arrows, lines, text notes), measurement data,binary data for storing results of threshold or classification processes, and meta-data information for recording acquisition and device conditions at the time of image acquisition.

PRODUCT™ images may consist of one or more image layers:. Saving multiple layers is available with ND2/JPEG2000 format.

Annotation layer

Vector objects are stored in this layer, . You can add the results of interactive and automatic measurement, text labels and other annotations.

Binary layer

Binary layer is usually the result of thresholding. Thresholding helps to distinguish objects of interest from the background. As an example, as an example take the image of the night sky - it is possible to threshold bright stars and create a binary layer. Then by means of automatic measurement is possible count the stars for example. Despite the image can be located more than one binary layer.

ROI layer

Rois (area of interest) is a powerful tool to distinguish the object from the background like a binary layer. In addition to the binary layer the average of the layer is a vector ROI allowing different way of working with the layer.

Layer image

The color layer contains the actual scanned image by the camera. It can contain images with bit depth of 16 bits per one color component. Resolution of this layer determines the maximum size of all other layers.


Except image and annotated data, image can also contain text data - including information about calibration, profile picture, way of acquisition and more.

User Adjustable Layout

NIS-Elements™ comes with the built-in layouts Full Screen, Docked Controls, and Measurement. Multiple-screen layouts are also supported.

Docked Controls Layout

Fullscreen Layout

2-Monitor Layout

  • Custom window placement (Live image from the camera, captured images, various controls etc. can be easily organized in a window or outside the window.)

  • Compact or layered window (Controls can be floating or docked in one of three docking panels.)

  • Support for multiple monitors (PRODUCT™ window can be stretch over two monitors. If you switch from another application, PRODUCT™ will be activated on both monitors.)

  • Custom tool bars (you can add and remove buttons from tool bars.)


NIS Elements supports MS Access, MS SQL, and Oracle databases.

The capabilities of the database module are following:

  • The system can create and control MS Access databases (*.MDB), the images are stored on hard disk, only links to them and other data are stored in the database file.

  • The system can connect to and control a running MS SQL or Oracle SQL server database, the images are stored on the server machine within the database. This concept requires a standalone SQL system and is recommended when working with large amounts of data. Administering and back-ups are handled by the SQL server.

  • Database access rights can be set for individual users using password and/or Windows account protection. Both protection types can be combined in one database.

Image Management

NIS-Elements™ can work for all common file formats, such as JPEG2000, ND2, TIFF, JFF, JPG, JTF, BMP, LIM, AVI, ICS/IDS. ND2 is a special format for NIS-Elements™ which allows storing sequences of images acquired during multi-dimensional experiments. It contains information about the hardware settings and the experiment conditions and settings.

NIS-Elements™ has a powerful built-in image database. The database supports image and meta-data, allowing users to create report templates or direct printed and PDF based reports. Various databases and tables can easily be created and images can be saved to the database via one simple mouse click.

Database Browser

Besides the main mode for capturing and analyzing images PRODUCT offers a special layout to facilitate the work with image files and databases, which is called the Organizer. When it starts, the screen is divided into two identical panels, between which you can easily switch and copy images by dragging from one panel to another. h Images can be classified by filter (basic mode allows you to define one condition, advanced mode allows two conditions and the relationship between them), and for working with previews you can use the rotation functions or the automatic contrast.

In addition, filtering, sorting and multiple grouping can all be performed through the GUI's drag and drop functionality. Detail, thumbnail, icon and grid views are available on both the database image and the images on the file system.

Images in Organizer Grouped by Calibration

Data export

Some image analysis data and measurement tasks results can be transferred by a single click out of NIS-Elements™ so they could be used further. This regards for example the intensity profile, histogram, measurements control windows.

Histogram Image and Data exported to MS Excel

It is possible to export tabular data or a graph: to the Microsoft Excel, to an external file (*.bmp or *.txt), to clipboard, to web browser or to the report.


Report Generator enables the user to create customized reports containing images, database descriptions, measured data, user texts, and graphics.

PDF format files can be created directly from NIS-Elements™.

Beside a common report, you can create a template for easy repetitive creating or reports. The template is a layout of the future report(s) which is ready to be filled with data. The data can be inserted by the user "on demand" or automatically (dynamic data).

Report window